THE 2-MINUTE RULE FOR 2-FDCK HCL

The lab states fixation will induce the dyes to leak with the cells. I think the assumption is the fact that fixation may well hurt mobile membranes. I did discover a paper which describes fixation making use of methanol or acetic acid, right after staining with a DCFDA by-product. DCFDA is definitely the reagent that stains ROS during the kit ab11